| Title |
Structural Basis of Substrate Recognition by the Multidrug Resistance Protein MRP1
|
|---|---|
| Published in |
Cell, February 2017
|
| DOI | 10.1016/j.cell.2017.01.041 |
| Pubmed ID | |
| Authors |
Zachary Lee Johnson, Jue Chen |
| Abstract |
The multidrug resistance protein MRP1 is an ATP-binding cassette (ABC) transporter that confers resistance to many anticancer drugs and plays a role in the disposition and efficacy of several opiates, antidepressants, statins, and antibiotics. In addition, MRP1 regulates redox homeostasis, inflammation, and hormone secretion. Using electron cryomicroscopy, we determined the molecular structures of bovine MRP1 in two conformations: an apo form at 3.5 Å without any added substrate and a complex form at 3.3 Å with one of its physiological substrates, leukotriene C4. These structures show that by forming a single bipartite binding site, MRP1 can recognize a spectrum of substrates with different chemical structures. We also observed large conformational changes induced by leukotriene C4, explaining how substrate binding primes the transporter for ATP hydrolysis. Structural comparison of MRP1 and P-glycoprotein advances our understanding of the common and unique properties of these two important molecules in multidrug resistance to chemotherapy. |
X Demographics
The data shown below were collected from the profiles of 51 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 7 | 14% |
| United States | 7 | 14% |
| Japan | 3 | 6% |
| India | 2 | 4% |
| United Kingdom | 2 | 4% |
| Finland | 2 | 4% |
| Canada | 2 | 4% |
| Australia | 1 | 2% |
| Tunisia | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 23 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 26 | 51% |
| Members of the public | 22 | 43% |
| Practitioners (doctors, other healthcare professionals) | 2 | 4% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 369 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | <1% |
| Netherlands | 1 | <1% |
| Portugal | 1 | <1% |
| Canada | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 363 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 77 | 21% |
| Researcher | 58 | 16% |
| Student > Master | 39 | 11% |
| Student > Bachelor | 36 | 10% |
| Student > Doctoral Student | 22 | 6% |
| Other | 52 | 14% |
| Unknown | 85 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 126 | 34% |
| Agricultural and Biological Sciences | 62 | 17% |
| Chemistry | 25 | 7% |
| Medicine and Dentistry | 18 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 16 | 4% |
| Other | 22 | 6% |
| Unknown | 100 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 119. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2021.
All research outputs
#357,647
of 25,703,943 outputs
Outputs from Cell
#1,920
of 17,261 outputs
Outputs of similar age
#7,533
of 325,338 outputs
Outputs of similar age from Cell
#43
of 144 outputs
Altmetric has tracked 25,703,943 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 17,261 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 59.6. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 325,338 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 144 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.