| Title |
Clonal Expansion of Lgr5-Positive Cells from Mammalian Cochlea and High-Purity Generation of Sensory Hair Cells
|
|---|---|
| Published in |
Cell Reports, February 2017
|
| DOI | 10.1016/j.celrep.2017.01.066 |
| Pubmed ID | |
| Authors |
Will J. McLean, Xiaolei Yin, Lin Lu, Danielle R. Lenz, Dalton McLean, Robert Langer, Jeffrey M. Karp, Albert S.B. Edge |
| Abstract |
Death of cochlear hair cells, which do not regenerate, is a cause of hearing loss in a high percentage of the population. Currently, no approach exists to obtain large numbers of cochlear hair cells. Here, using a small-molecule approach, we show significant expansion (>2,000-fold) of cochlear supporting cells expressing and maintaining Lgr5, an epithelial stem cell marker, in response to stimulation of Wnt signaling by a GSK3β inhibitor and transcriptional activation by a histone deacetylase inhibitor. The Lgr5-expressing cells differentiate into hair cells in high yield. From a single mouse cochlea, we obtained over 11,500 hair cells, compared to less than 200 in the absence of induction. The newly generated hair cells have bundles and molecular machinery for transduction, synapse formation, and specialized hair cell activity. Targeting supporting cells capable of proliferation and cochlear hair cell replacement could lead to the discovery of hearing loss treatments. |
X Demographics
The data shown below were collected from the profiles of 44 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 25% |
| Japan | 2 | 5% |
| Russia | 2 | 5% |
| United Kingdom | 2 | 5% |
| India | 1 | 2% |
| Italy | 1 | 2% |
| Pakistan | 1 | 2% |
| Canada | 1 | 2% |
| China | 1 | 2% |
| Other | 7 | 16% |
| Unknown | 15 | 34% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 33 | 75% |
| Scientists | 7 | 16% |
| Practitioners (doctors, other healthcare professionals) | 2 | 5% |
| Science communicators (journalists, bloggers, editors) | 2 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 231 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 231 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 52 | 23% |
| Student > Ph. D. Student | 44 | 19% |
| Student > Bachelor | 23 | 10% |
| Student > Master | 22 | 10% |
| Other | 13 | 6% |
| Other | 26 | 11% |
| Unknown | 51 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 52 | 23% |
| Agricultural and Biological Sciences | 38 | 16% |
| Medicine and Dentistry | 24 | 10% |
| Neuroscience | 22 | 10% |
| Engineering | 8 | 3% |
| Other | 27 | 12% |
| Unknown | 60 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 473. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2023.
All research outputs
#58,170
of 25,827,956 outputs
Outputs from Cell Reports
#72
of 13,279 outputs
Outputs of similar age
#1,323
of 427,556 outputs
Outputs of similar age from Cell Reports
#2
of 262 outputs
Altmetric has tracked 25,827,956 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,279 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 30.2. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 427,556 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 262 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.